Cell Cycle and Mitosis

The cell cycle profile of a sample is an important indicator of whether normal or abnormal proliferation is taking place. In addition to the cell cycle profile typical to flow cytometry, the ImageStream combines high speed multispectral image acquisition and automated image analysis to allow identification of the morphologically distinguishable phases of mitosis as well as localization of molecules of interest during each phase of the cell cycle.

Cell Cycle Measurement using the ImageStream

In addition to the cell cycle analysis based on DNA content, image analysis features can be used to distinguish mitotic events from G2 cells based on nuclear morphology. See the application note for more details.