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Featured Application
Mitotic Index Multiplexed with Apoptotic Cell Detection
Accurate and Reliable Multi-parametric Analysis of Apoptosis
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Simultaneous Measurement of NF-kB Translocation and Apoptosis
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Quantitation of Nuclear Translocation Events Using ImageStream Multispectral Imaging Cytometry
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Analysis of Apoptosis by Nuclear Fragmentation
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Quantitative Analysis of Pseudopod Formation
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Distinguishing Modes of Cell Death Using ImageStream Multispectral Imaging Cytometry
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Multiplexed Analysis of Cells and Beads with the ImageStream Imaging Flow Cytometer
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Analysis of Intracellular Molecular Trafficking Using ImageStream Multispectral Imaging
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Analysis of Complement 3b Deposition on Rituximab Opsonized Cells Using ImageStream Multispectral Imaging Cytometry
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GFP Expression and Subcellular Compartmentalization in Trypanosomes
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Posters
Automated Classification Of Apoptosis And Artifact Rejection Of Tunel Positive Cells
Presented at ISAC XXIII, May 2006
Image Data Exploration And Analysis Software
Presented at ISAC XXIII, May 2006
Assessment Of Protein Co-Localization With An Imaging Flow Cytometer And Novel Analytical Method
Presented at ISAC XXIII, May 2006
StreamLine eNewsletters
2007 Newsletters
2006 Newsletters
- StreamLine Volume 3 No. 4
- StreamLine Volume 3 No. 3
- StreamLine Volume 3 No. 2
- StreamLine Volume 3 No. 1
Press Releases
Amnis Announces Release of Turbo Upgrade to the Imagestream® System
View Article
Imagestream® Used For Pioneering Advance in AIDS Research
View Article
Featured Publication
Temporal Dissection of T-bet Functions
J Immunol. (2007) 178: 3457-65
Additional Publications
The Doublecortin-expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to
Neuronal-lineage Cells.
The Journal of Neuroscience (2007) 27: 3734-42
Link to publication
NKT Cell Activation Mediates Neutrophil IFN-g Production and Renal Ischemia-Reperfusion Injury.
The Journal of Immunology (2007) 178: 5899-911.
Link to publication
Aryl Hydrocarbon Receptor Activation during Influenza Virus Infection Unveils a Novel Pathway of IFN-gamma Production by
Phagocytic Cells.
The Journal of Immunology (2007) 179: 247-55.
Link to publication
Regulation of NF-kB Activation in T Cells via Association with the Adaptor Proteins ADAP and CARMA1
Science (2007) 316: 754-8
Link to publication
Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells
Science (2007) 315: 1398-1401
Link to publication
Extended depth of field imaging for high speed cell analysis
Cytometry Part A (2007) 71:215-31
Link to publication
Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging cytometer
Journal of Immunological Methods. (2006) 317:90-99.
Link to publication
Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-Alpha Production
The Journal of Immunology (2006)
177: 5829-5839.
Link to publication
Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-throughput Single-Cell Analysis of Kaposi's Sarcoma-Associated Herpesvirus Infection
Journal of Virology (2006) 80:10073-10082.
Link to publication
Sensitivity measurement and compensation in spectral imaging
Cytometry Part A (2006) 69: 852-862.
Link to publication
KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
Journal of Clinical Investigation
(2006) 116: 1963-73.
Link to publication
Features of Medullary Thymic Epithelium Implicate Postnatal Development in Maintaining Epithelial Heterogeneity and Tissue-Restricted Antigen Expression
The Journal of Immunology (2006) 176: 5815-5824
Link to publication
Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow.
Journal of Immunological Methods (2006) 311:117-29.
Link to publication
Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies in Flow Cytometry for Biotechnology
Link to publication
Cutting Edge: FADD Is Not Required for Antigen Receptor-Mediated NF-κB Activation
The Journal of Immunology (2005) 175: 7800-7804
Link to publication
Upcoming Events
September 28-30, 2007
GLIIFCA
Windsor, Ontario
Canada
October 7-9, 2007
Clinical Cytometry Society
Washington, DC
Booth No. 208
October 10-13, 2007
German Society for Flow Cytometry
Regensburg, Germany
December 1-5, 2007
American Society for Hematology
Atlanta, GA
Booth No. 1347
Special Event
Register now for this important event.
Scientific presentations by users will introduce you to the broad range of research applications enhanced or enabled by the ImageStream system.
Amnis scientists will present the latest developments and discuss future directions for the technology.
The visual power of microscopy and the statistical rigor of flow
cytometry are now combined in a single platform.
What can Multispectral Imaging Flow Cytometry on the ImageStream do
for you? The powerful combination of quantitative image analysis and
flow cytometry in a single platform creates exceptional new experimental
capabilities. Measure the amount, location and movement of molecules
on, in, or between cells. Measure the location and co-localization of
multiple markers on or in cells. Quantitate morphologically distinct
cell subpopulations. Dramatically expand the depth and range of questions
you can ask in a flow environment.
When you analyze your cells on the ImageStream you will see more – and know more – than you ever thought possible.
Find out more about ImageStream technology
View ImageStream eBrochure
ImageStream FAQ
IDEAS® Image Analysis Software: exceptional accessible power
Just because an image analysis package is powerful doesn’t mean it should be hard to use.
IDEAS cell image analysis software gets you to your data quickly and easily
and gives you a rich suite of tools to analyze almost any problem. The
continually expanding IDEAS feature set offers over 250 features, many
developed just for the ImageStream system. 
- Every dot in a scatter plot is linked to a cell image – click on the dot and you will see the corresponding cell
- Virtual Cell Sorting – use familiar gating tools to define and inspect cell populations
- Image data and statistical data are fully integrated – complete statistics are always available for every population
- Powerful built-in algorithms calculate similarities between different images of the same cell -- colocalize signals without FRET!
One Instrument, Unlimited Applications
Many new applications are enabled by the ImageStream system, and many existing flow cytometry applications are dramatically improved. The following are just a few of the many ways in which the ImageStream can bring more power and insight to your research.
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| Cell Classification | Nuclear Translocation | Apoptosis | Surface Marker Colocalization |
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| Cell Conjugates | Pseudopod Formation | Phagocytosis | Molecular Trafficking |
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| FISH in Suspension | Gene Expression | Immune Synapse | Toxoplasma Gondii |
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For a complete listing of technical reports, application notes and publications, please visit our Applications page.
Expand the power of your ImageStream system with these new upgrades
Multiple Lasers
Use a wider range of dyes and enable even more applications by adding
up to two additional lasers to the basic system. In addition to the
standard 488nm laser, you can now add a 375nm, a 405nm or a 658nm. The
lasers may be ordered installed on your ImageStream as field upgrades.
Click to receive more information about the Multilaser upgrade
Extended Depth of Field
Remarkable new optical deconvolution technology allows the ImageStream
depth of field to be expanded more than 5-fold. Enabling such applications
as FISH In Suspension, extended depth of field also markedly improves
many resolution-sensitive applications.
Click to receive more information about the Extended Depth of Field
upgrade
IDEAS® Software Tutorials Now Available Online
Amnis has prepared a library of online tutorials to support the IDEAS image analysis software package.
Each tutorial covers a specific topic and may be viewed online without need to download.
Available topics include:
Opening a Raw Image File
Compensation
Image Gallery Display
Colocalization
Nuclear Translocation
See the full online tutorial library
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