The study of DNA repair mechanisms is important in the field of oncology where radiotherapy used to treat tumors induces double strand breaks (DSB).The DNA damage that occurs can be indirectly visualized with a microscope by immuno-staining the repair proteins that are recruited to DSB foci. The ImageStream automatically and objectively collect thousands of images, quantifies the foci in a population of cells and determines dose response kinetics faster and easier than manual microscopy.
Phosphorylated H2AX (γ-H2AX) facilitates recognition and repair of DNA double strand breaks (DSBs) that may occur from exposure to ionizing radiation. Staining irradiated cells for γ-H2AX reveals nuclear foci that are readily observed microscopically in a dose response manner. Irradiated cells were analyzed for the number of spots in the nuclear region using advanced masking techniques that identify the punctate staining. Morphological measurements employed in this analysis including object shape, size, and punctate fluorescence spot counting emphasizing the advantages of quantitative multiparametric image analysis on large numbers of cells provided with the ImageStream.
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