The
Stream Line was designed to keep you up to date with the applications and technical
developments related to the Amnis ImageStreamŽ System, the revolutionary new
technology for imaging in flow. You will also find information on how to contact
Amnis and where you will be able to see ImageStream technology firsthand. For
further information, please visit the Amnis website at www.amnis.com
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Analysis
of the major subpopulations of human leukocytes -- monocytes, lympnocytes, eosinophils,
neutrophils, and basophils -- is a fundamental procedure in hematology. Here,
we demonstrate the capacity of the ImageStream
100 to perform a full five-part differential analysis of human leukocytes
using only a fluorescent stain for CD45, a DNA binding dye and the morphological
information inherent to the cells themselves. This highly efficient and quantitative
analysis lays a foundation for more extensive analysis using additional stains
or fluorescent probes.
The newly expanded and
updated Applications section at www.amnis.com demonstrates the value of the
ImageStream system in ten distinct application categories with numerous specific
examples of experiments and assays.
Amnis Corporation
2505 Third Avenue, Suite 210
Seattle, WA 98121
1-800-730-7147
www.amnis.com
Copyright © 2004 Amnis Corporation All Rights Reserved
Achieving
adquate sensitivity was one of the major challenges in developing imaging-in-flow
technology. Most fluorescent signals are too dim for imaging with standard CCD
approaches. Amnis solved the problem using a unique CCD camera and an innovative
data collection technology called Time Delay Integration. This report describes
in detail how TDI works to give you high resolution cell images at sensitivities
equal to -- or better than -- conventional flow cytometry.