IDEAS Software Tutorials
Are Now Available Online

Amnis has prepared a library of online tutorials to support the IDEAS image analysis software package.

Each tutorial covers a specific topic and may be viewed online without need to download.

The tutorials are grouped into two general categories: Basic Functions and Applications. The Basic Functions tutorials are designed to reinforce instrument training or introduce new users to selected analytical techniques.

Examples include:

Opening a Raw Image File

Compensation

Image Gallery Display

See more Basic Function tutorials

The Applications tutorials are typically larger and cover the details of analyzing a particular type of sample.

Examples here include:

Colocalization

Nuclear Translocation

See the full online tutorial library

Upcoming Events

WNYFUG
Western NY Flow Users Group
University of Rochester
Rochester, NY
July 12, 2006

SBS 2006
Society for Biomolecular Screening
Washington State Convention Center
Seattle, WA
September 17-21, 2006

Clinical Cytometry
Clinical Cytometry Society
Hyatt Regency
Long Beach, CA
October 8-10, 2006

ASH 2006
American Society of Hematology
Orange County Convention Center
Orlando, FL
December 9-11, 2006


Miami Winter Symposium
Miami Winter Symposium
Miami Beach Resort & Spa Hotel
Miami, FL
January 27-31, 2007

The Stream Line
June 19, 2006
Vol 3 Number 2

The Stream Line is a free, monthly email publication designed to keep you up to date with the applications and technical developments related to the Amnis ImageStream® System. For further information, please visit the Amnis website at www.amnis.com

Copyright © 2006 Amnis Corporation All Rights Reserved

These improvements expand the power of the ImageStream system, allowing more samples, more applications and significantly greater reliability. The system is now easier to use, more flexible and offers a wider array of functions.

Overall, the improvements provide a 3-5X increase in the speed of data collection, allowing many more samples to be run in a day. Sensitivity has improved by more than a factor of two and extensive engineering changes have significantly improved the system's reliability. Finally, a large number of modifications to the IDEAS and INSPIRE software packages make the ImageStream even easier and more intuitive to operate. The Turbo upgrade is now standard on all ImageStream systems.

For a detailed review of Turbo improvements, click here.

RESEARCH REPORT

Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow.
George, TC¹, Fanning, SL², Fitzgeral-Bocarsley P², Medeiros RB³, Highfill S³, Shimizu Y³, Hall BE¹, Frost K¹, Basiji D¹, Ortyn WE¹, Morrissey PJ¹, and Lynch DH¹.
J Immunological Methods 2006 Apr 20;311(1-2):117-129

1 Amnis Corporation, Seattle, WA
2 Graduate School of Biomedical Sciences, University of Medicine and Dentistry of New Jersey, Newark NJ
3 Department of Laboratory Medicine and Pathology, Center for Immunology, Cancer Center, University of Minnesota Medical School, Minneapolis, MN

Nuclear translocation of NF-kB initiates transcription of numerous genes, many of which are critical to host defense. Fluorescence image-based methods that quantify this event have historically used adherent cells with large cytoplasm-to-nuclear area ratios. Using the ImageStream imaging flow cytometer, we have developed a novel method that measures nuclear translocation in large populations using cross-correlation analysis of nuclear and NF-kB images from each cell.

The approach presented here accurately measures NF-kB translocation in cells iwth small cytoplasmic areas in dose- and time-dependent manners. Further, NF-kB translocation was accurately measured in a subset of cells contained in a mixed population and the technique was successfully employed to measure IRF-7 translocation in plasmacytoid dendritic cells (PD) obtained from peripheral blood.

Please click here to view the abstract and/or publication details.