Amnis Corporation - The StreamLine

Explore a wealth of technical literature on the Amnis web site . . .

If you're interested in technical information about the ImageStream system, you'll find the Downloads page on the Amnis web site to be exceptionally valuable.

The Downloads page is designed to get you all the information you need, quickly and easily and in one place.

Contents of the Downloads page include:

Application Notes

Publications

Posters from Scientific Meetings

Technology Reports

Product Literature

The Downloads page is updated regularly, so be sure to check back for new additions to the literature listings.

Upcoming Events

Clinical Cytometry
Clinical Cytometry Society
Hyatt Regency
Long Beach, CA
October 8-10, 2006

Cytometrie 2006
Oud Sint-jan
Brussels, Belgium
October 18-20, 2006

NIH Research Festival
NIH Campus
Bethesda, MD
October 19-20, 2006

AIC
Autumn Immunology Conference Chicago, IL
November 17-20, 2006

ASH 2006
American Society of Hematology
Orange County Convention Center
Orlando, FL
December 9-11, 2006

Miami Winter Symposium
Miami Winter Symposium
Miami Beach Resort & Spa Hotel
Miami, FL
January 27-31, 2007

The Stream Line
September 24, 2006
Vol 3 Number 4

The Stream Line is a free, monthly email publication designed to keep you up to date with the applications and technical developments related to the Amnis ImageStream® System. For further information, please visit the Amnis website at www.amnis.com

New Publication

Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell
Analysis of Kaposi’s Sarcoma-Associated Herpesvirus Infection

In this publication, Drs. Laura Adang, Christopher Parsons and Dean Kedes report on the analysis of KSHV infection using the ImageStream system.

In the October, 2006 issue of the Journal of Virology, the authors report their studies using the KSHV nuclear antigen LANA to quantitate viral load. The viral protein measurements correleate well with viral DNA concent and provide a valuable new method for understanding of the virus’s biology and pathogenesis. According to the authors, use of the ImageStream system "specifically and sensitively identifies infected cells even if they are present at low frequencies and further distinguishes these cells based on the level of infection."

Research Report

Accurate quantitation of nuclear fragmentation provides a valuable perspective on apoptosis

Nuclear fragmentation is a morphologic feature with significant value in the evaluation of apoptotic states, but is difficult to measure for large cell numbers. The ImageStream system combines high resolution microscopy with the capacity to analyze large populations, making the use of nuclear fragmentation a more powerful metric of apoptosis.

The most reliable distinguishing feature of the apoptotic process is nuclear condensation and DNA fragmentation. Apoptotic nuclei stained with fluorescent DNA intercalating dyes typically produce small, fragmented, highly textured nuclear images. Because of this distinctive morphology, apoptotic nuclei are ideally suited for analysis with the ImageStream system.

View or download the full report.

For the Flow Cytometry Core Facility

A flow cytometry core facility must meet the requirements of many investigators. Versatility and technology can be critical to fulfilling the mission of the core facility. In this presentation, Greg Veltri describes how the ImageStream has improved the ability of his facility to provide exceptional analytical breadth.

Technology Publication

ImageStream technology demonstrates high sensitivity and dynamic range, plus effective compensation of complex multimode imagery.

The ImageStream system combines advances in CCD technologies with a novel optical architecture for high sensitivity and multispectral imaging of cells in flow. The sensitivity and dynamic range as well as a methodology for spectral compensation of imagery is presented in this peer-reviewed publication.