Imaging and Analysis of Cells in Flow

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Product Literature

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Users Guides and Manual

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  • ImageStream® QuickStart Guide
  • INSPIRE™ Instrument Software User Guide
  • IDEAS™ Image Analysis Software User Guide
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Application Notes

Apoptosis

Distinguishing modes of cell death using ImageStream Multispectral Imaging Spectroscopy

Advances in the identification of apoptotic cells

Quantitation of Apoptosis by Nuclear Fragmentation

Nuclear Translocation

Quantitation of Nuclear Translocation Events Using ImageStream Multispectral Imaging Spectroscopy

Simultaneous Measurement of Nf-kB Translocation and Apoptosis with the ImageStream Multispectral Cell Imaging System

Leukocyte Classification

Multiplexed Analysis of Cells and Beads with the ImageStream Imaging Flow Cytometer

Marker Co-localization

Analysis of Complement 3b deposition on Rituximab opsonized cells using ImageStream® Multispectral Imaging Cytometry

Quantitative Analysis of Cell Morphology

Quantitative Analysis of Pseudopod Formation with the ImageStream® Cell Imaging System

Intracellular Molecular Trafficking

Analysis of Intracellular Molecular Trafficking using ImageStream® Multispectral Imaging

Quantitation of Gene Expression

GFP Expression and Subcellular Compartmentalization in Trypanosomes: Similarity Score Analysis on the ImageStream® Cell Analysis System

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Publications

Extended depth of field imaging for high speed cell analysis
William E Ortyn, David J Perry, Vidya Venkatachalam, Luchuan Liang, Brian E Hall, Keith Frost, David A Basiji
Cytometry, Part A 2007 Apr; 71(4):215-31

Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells
Heung Kyu Lee, Jennifer M. Lund, Balaji Ramanathan, Noboru Mizushima, Akiko Iwasaki
Science DOI: 10.1126/science.1136880 2007


Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging cytometer
Paul V. Beum, Margaret A. Lindorfer, Brian E. Hall, Thaddeus C. George, Keith Frost, Philip J. Morrissey, Ronald P. Taylor. Journal of Immunological Methods 2006 317:90-99

 

Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-Alpha Production
Stacey L. Fanning, Thaddeus C. George, Di Feng, Steven B. Feldman, Nicholas J. Megjugorac, Alexander G. Izaguirre, and Patricia Fitzgerald-Bocarsly. The Journal of Immunology 2006 177: 5829-5839

Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell Analysis of Kaposi's Sarcoma-Associated Herpesvirus Infection
Laura A. Adang, Christopher H. Parsons, and Dean H. Kedes. Journal of Virology 2006 80:10073-10082

Sensitivity measurement and compensation in spectral imaging
WE Ortyn, BE Hall, TC George, K Frost, DA Basiji, DJ Perry, CA Zimmerman, D Coder, PJ Morrissey. Cytometry PartA 69A: 852-862 (2006)

KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
CH Parsons, LA Adang, J Overdevest, CM O'Connor, JR Taylor Jr, D Camerini and DH Kedes. Journal of Clinical Investigation doi:10.1172/JCI27249 (2006)

Features of Medullary Thymic Epithelium Implicate postnatal Development in Maintaining Epithelial Heterogeneity and Tissue-Restricted Antigen Expression
GO Gillard, AG Farr Journal of Immunology 2006, 176: 5815-5824

Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow.
T.C. George, S.L. Fanning, P. Fitzgerald-Bocarsly, R.B. Medeiros , S.Highfill, Y.Shimizu, B.E. Hall, K.Frost, D.A.Basiji, W.E. Ortyn, P.J. Morrissey, D.H. Lynch Journal of Immunological Methods 2006 Apr 20;311(1-2):117-29.

Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies in Flow Cytometry for Biotechnology
Larry A. Sklar, Editor. Oxford University Press, 2005.

Flow Cytometry, Smaller and Better.
L. Bonetta. Nature Methods 2(10), 785-95 (2005).

Cutting Edge: FADD Is Not Required for Antigen Receptor-Mediated NF-kB Activation.
A. F. Arechiga, B.D. Bell, J. C. Solomon, I.H. Chu, C.L. Dubois, B. E. Hall, T.C. George, D.M. Coder, C.M. Walsh. Journal of Immunological Methods. 175, 7800-04 (2005)

Distinguishing Modes of Cell Death Using the ImageStream Multispectral Imaging Spectrometer.
T.C. George, D.A. Basiji, B.E. Hall, D.H. Lynch, W.E. Ortyn, D.J. Perry, M.J. Seo, C.A. Zimmerman, P.J. Morrissey Cytometry 59A, 237-45 (2004)

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posters

ISAC XXIII
May, 2006

Automated classification of apoptosis and artifact rejection of tunel positive cells
poster Number P193
Abstract Number 150560

Image data exploration and analysis software
poster Number P 212
Abstract Number 150703

Assessment of protein co-localization with an imaging flow cytometer and novel analytical method
poster Number P244
Abstract Number 150483

Clinical Cytometry Society
October, 2005

Multiplexed cell classification and cytometric bead array analysis using the ImageStream100 imaging cytometer

American Society for Hematology
December, 2004

Cell Classification in Human Peripheral Blood using the Amnis ImageStream® Flow Imaging System

ISAC XXII
May, 2004

Distinguishing Modes of Cell Death Using ImageStream® Multispectral Imaging Cytometry

Multispectral High Content Cellular Analysis Using a Flow Based Imaging Cytometer

Fluorescent In Situ Hybridization In Suspension Analysis Using ImageStream® Multispectral Imaging Flow Cytometry

Quantitation of Nuclear Translocation Events Using ImageStream® Multispectral Imaging Cytometry

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Technology Reports

Time Delay Integration: enabling high sensitivity detection for Imaging-in-Flow on the ImageStream® Cell Analysis System

The IDEAS™ Similarity Score: A Powerful Tool for Comparative Image Analysis with the ImageStream® System

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StreamLine eNewsletters

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