Image the Whole Cell in Focus

The extended depth of field (EDF™) option incorporates Wavefront Coding™ technology from CDM Optics, which is a combination of specialized optics and unique image processing algorithms, to project all structures within the cell into one crisp plane of focus.

EDF technology dramatically extends the depth of field, enabling new applications and improving the performance of existing applications – especially spot counting applications such as FISH, autophagy, and nuclear translocation.

Figure 1 shows the effect of progressive defocus on a test pattern of 2μ bars. Figure 2 shows how the same pattern imaged with EDF technology maintains excellent image quality over the entire focus range.