Multispectral Imaging

The multispectral imaging system acquires up to 12 images per cell in three different imaging modes: brightfield, darkfield, and fluorescence. Light is collected from the cells flowing in the cuvette with custom objective lenses and relayed to the spectral decomposition element, which consists of a custom set of longpass filters in an angular array. The filters direct different spectral bands to laterally distinct channels on the CCD detector. The cell images are collected using patent time-delay integration (TDI) technology which yields fluorescence sensitivity comparable to the best flow cytometers. With this technique, an image is optically decomposed into a set of sub-images, each corresponding to a different color component and spatially isolated from the remaining sub-images.